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Journal Articles Journal of Pharmacological and Toxicological Methods Year : 2013

Accelerated Caco-2 cell permeability model for drug discovery

Abstract

Introduction: By culturing Caco-2 cells according to a new and optimized protocol, it has been possible to accelerate the cell culture process in such a way that the cells can be used for experiments after only 6 days. The accelerated Caco-2 model has been compared to the traditional model (requiring 21-25 days of culture) in terms of tightness of the junctions, ability to rank chemical compounds for apparent permeability, active efflux and to discriminate P-gp substrates. Methods and results: In the new protocol, Caco-2 cells were cultured with the classical Caco-2 medium supplemented with puromycin. The initial cell seeding density was increased two times compared to the traditional procedure and the presence of a low concentration of puromycin in the culture medium reduced the Caco-2 permeability of mannitol. Bi-directional studies were performed with known P-gp substrates (rhodamine 123, digoxin and saquinavir) and with a total of 20 marketed drugs covering a wide range of physicochemical characteristics and therapeutic indications. Strong correlations were obtained between the apparent permeability in absorptive (Papp A→B) or secretory (Papp B→A) of the drugs in the accelerated model and in the traditional models and comparable efflux ratios were observed in the two studied models. Discussion: The new protocol reduces costs for screening and leads to higher throughput compared to traditional Caco-2 cell models. This accelerated model provides short time-feedback to the drug design during the early stage of drug discovery.
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Dates and versions

hal-02515314 , version 1 (23-03-2020)

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Emmanuel Sevin, L. Dehouck, A. Fabulas-da Costa, R. Cecchelli, M.P. Dehouck, et al.. Accelerated Caco-2 cell permeability model for drug discovery. Journal of Pharmacological and Toxicological Methods, 2013, 68 (3), pp.334-339. ⟨10.1016/j.vascn.2013.07.004⟩. ⟨hal-02515314⟩

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