Cross-talk between brain capillary endothelial cells and astrocytes: upregulation of the low density lipoprotein receptor at the blood-brain barrier - Université d'Artois Access content directly
Journal Articles Nutrition, Metabolism and Cardiovascular Diseases Year : 1997

Cross-talk between brain capillary endothelial cells and astrocytes: upregulation of the low density lipoprotein receptor at the blood-brain barrier

Abstract

In contrast to the endothelial cells in large vessels where low density lipoprotein receptors are down-regulated, brain capillary endothelial cells in vivo express an LDL receptor. Using a cell culture model of the blood-brain barrier consisting of a coculture of brain capillary endothelial cells and astrocytes, we observed that the capacity of endothelial cells to bind LDL is enhanced 3 fold when cocultured with astrocytes. We next investigated the ability of astrocytes to modulate endothelial cell LDL receptor expression. We have shown that the lipid requirement of astrocytes increases the expression of endothelial cell LDL receptors. Experiments with dialysis membranes of different pore size showed that this effect is mediated by a soluble factor(s) with a molecular mass somewhere between 3,500 and 14,000. Substituting astrocytes with smooth muscle cells or brain endothelium with endothelium from the aorta or the adrenal cortex did not enhance the luminal LDL receptor expression on endothelial cells, demonstrating the specificity of the interactions. This study demonstrates that a cross-talk takes place between brain capillary endothelial cells and astrocytes.
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Dates and versions

hal-00546733 , version 1 (14-12-2010)

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  • HAL Id : hal-00546733 , version 1

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Bénédicte Dehouck, Laurence Tilloy-Fenart, Marie-Pierre Dehouck, Roméo Cecchelli, Jean-Charles Fruchart. Cross-talk between brain capillary endothelial cells and astrocytes: upregulation of the low density lipoprotein receptor at the blood-brain barrier. Nutrition, Metabolism and Cardiovascular Diseases, 1997, 7, p. 225-230. ⟨hal-00546733⟩
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